Evaluation of different strategies for the use of ELISA tests as first screening tools for serologic surveillance of low pathogenic avian influenza in the Belgian poultry sector.
Identifieur interne : 000072 ( Main/Exploration ); précédent : 000071; suivant : 000073Evaluation of different strategies for the use of ELISA tests as first screening tools for serologic surveillance of low pathogenic avian influenza in the Belgian poultry sector.
Auteurs : Sylvie Marché [Belgique] ; Thierry Van Den BergSource :
- Avian diseases [ 0005-2086 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Belgique, Grippe chez les oiseaux (virologie), Grippe chez les oiseaux (épidémiologie), Sensibilité et spécificité, Test ELISA (), Test ELISA (médecine vétérinaire), Trousses de réactifs pour diagnostic (médecine vétérinaire), Virus de la grippe A (isolement et purification), Virus de la grippe A (pathogénicité), Volaille, Études séroépidémiologiques.
- MESH :
- isolement et purification : Virus de la grippe A.
- médecine vétérinaire : Test ELISA, Trousses de réactifs pour diagnostic.
- pathogénicité : Virus de la grippe A.
- virologie : Grippe chez les oiseaux.
- épidémiologie : Grippe chez les oiseaux.
- Animaux, Belgique, Sensibilité et spécificité, Test ELISA, Volaille, Études séroépidémiologiques.
- Wicri :
- geographic : Belgique.
English descriptors
- KwdEn :
- Animals, Belgium, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (veterinary), Influenza A virus (isolation & purification), Influenza A virus (pathogenicity), Influenza in Birds (epidemiology), Influenza in Birds (virology), Poultry, Reagent Kits, Diagnostic (veterinary), Sensitivity and Specificity, Seroepidemiologic Studies.
- MESH :
- chemical , veterinary : Reagent Kits, Diagnostic.
- geographic : Belgium.
- epidemiology : Influenza in Birds.
- isolation & purification : Influenza A virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- pathogenicity : Influenza A virus.
- veterinary : Enzyme-Linked Immunosorbent Assay.
- virology : Influenza in Birds.
- Animals, Poultry, Sensitivity and Specificity, Seroepidemiologic Studies.
Abstract
Since the emergence of the highly pathogenic avian influenza H5N1, avian influenza surveillance has been expanded in Europe. The serologic monitoring of domestic poultry is usually accomplished using the reference hemagglutination inhibition (HI) test for the detection of H5 and H7 subtypes. However, as the number of tested sera has been increasing, there is a need for another serologic method that could be used as a preliminary screening test. A comparison of four enzyme-linked immunosorbent assay (ELISA) tests (two indirect and two competitive) was conducted, and they showed good specificity and higher sensitivity than the HI test. The selected ELISA tests were then tested using approximately 800 field sera representative of different poultry species, and a simulation was done to determine the best strategy for screening. The first strategy was testing both gallinaceous and nongallinaceous sera with a competitive ELISA and using the HI test for H5 and H7 as a confirmatory test. The second strategy was testing only gallinaceous bird sera with the indirect ELISA with confirmatory H5 and H7 HI and all nongallinaceous sera by the H5 and H7 HI test. In the Belgian poultry context, the best strategy seems to be the use of a blocking ELISA as the primary screening tool to test all the poultry sera, followed by confirmation by H5 and H7 HI test subtyping.
DOI: 10.1637/8759-033109-Reg.1
PubMed: 20521705
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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xml:lang="en">Since the emergence of the highly pathogenic avian influenza H5N1, avian influenza surveillance has been expanded in Europe. The serologic monitoring of domestic poultry is usually accomplished using the reference hemagglutination inhibition (HI) test for the detection of H5 and H7 subtypes. However, as the number of tested sera has been increasing, there is a need for another serologic method that could be used as a preliminary screening test. A comparison of four enzyme-linked immunosorbent assay (ELISA) tests (two indirect and two competitive) was conducted, and they showed good specificity and higher sensitivity than the HI test. The selected ELISA tests were then tested using approximately 800 field sera representative of different poultry species, and a simulation was done to determine the best strategy for screening. The first strategy was testing both gallinaceous and nongallinaceous sera with a competitive ELISA and using the HI test for H5 and H7 as a confirmatory test. The second strategy was testing only gallinaceous bird sera with the indirect ELISA with confirmatory H5 and H7 HI and all nongallinaceous sera by the H5 and H7 HI test. In the Belgian poultry context, the best strategy seems to be the use of a blocking ELISA as the primary screening tool to test all the poultry sera, followed by confirmation by H5 and H7 HI test subtyping.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20521705</PMID><DateCompleted><Year>2010</Year><Month>07</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0005-2086</ISSN><JournalIssue CitedMedium="Print"><Volume>54</Volume><Issue>1 Suppl</Issue><PubDate><Year>2010</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Avian diseases</Title><ISOAbbreviation>Avian Dis.</ISOAbbreviation></Journal><ArticleTitle>Evaluation of different strategies for the use of ELISA tests as first screening tools for serologic surveillance of low pathogenic avian influenza in the Belgian poultry sector.</ArticleTitle><Pagination><MedlinePgn>627-31</MedlinePgn></Pagination><Abstract><AbstractText>Since the emergence of the highly pathogenic avian influenza H5N1, avian influenza surveillance has been expanded in Europe. 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The second strategy was testing only gallinaceous bird sera with the indirect ELISA with confirmatory H5 and H7 HI and all nongallinaceous sera by the H5 and H7 HI test. 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